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Molecular Serotyping and Characterization of Shiga Toxin-Producting E. Coli (Stec)

Objective

To develop DNA-based typing/characterization platforms for Shiga toxin-producing E. coli.

More information

Approach:
Sequencing of the Escherichia coli O antigen gene clusters and development of genetic-based typing and detection systems. It will address the 60-month milestone for this project, Complete studies on development of genetic-based systems for typing E. coli and detection of non-O157 STEC, and develop methods for isolation of non-O157 STEC. This agreement will expand on our current research and will result in the development of methods to identify, detect, and characterize E. coli O157:H7 and non-O157 STEC, a critical need of regulatory agencies and the food industry.

Investigators
Yan, Xianghe; Fratamico, Pina
Institution
USDA - Agricultural Research Service
Start date
2009
End date
2013
Project number
1935-42000-070-01
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