To develop DNA-based typing/characterization platforms for Shiga toxin-producing E. coli.
Approach:
Sequencing of the Escherichia coli O antigen gene clusters and development of genetic-based typing and detection systems. It will address the 60-month milestone for this project, Complete studies on development of genetic-based systems for typing E. coli and detection of non-O157 STEC, and develop methods for isolation of non-O157 STEC. This agreement will expand on our current research and will result in the development of methods to identify, detect, and characterize E. coli O157:H7 and non-O157 STEC, a critical need of regulatory agencies and the food industry.