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National Agricultural Library
U.S. Department of Agriculture
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  4. A NOVEL APPROACH TO INVESTIGATE INTERNALIZATION OF ESCHERICHIA COLI O157:H7 IN LETTUCE AND SPINACH

A NOVEL APPROACH TO INVESTIGATE INTERNALIZATION OF ESCHERICHIA COLI O157:H7 IN LETTUCE AND SPINACH

Objective

1. Develop strains of Escherichia coli O157:H7 and non-pathogenic E. coli that contain the gfp gene inserted into the bacterial chromosome. 2. Determine the survival and growth of gfp-labeled wild type and rpoS-deficient E. coli O157:H7 populations in internalized tissues. 3. Determine differences in the expression of virulence factors in E. coli O157:H7 grown on leafy greens and ground beef.

Investigators
Sharma, Vijay
Institution
USDA - Agricultural Research Service
Start date
2007
End date
2010
Funding Source
Agricultural Research Service
Project number
1265-32420-004-05T
Accession number
411923
Categories
Escherichia coli
Viruses and Prions
Bacterial Pathogens
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