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This research explores the application of different analytical methods (non-PCR based) for the detection of GM materials.
<p>The range of techniques proposed focus on both alternative DNA amplification and detection technologies.
<p>The programme is divided into the investigation of two of these techniques:<ul>
<li>rolling circle amplification (RCA) – isothermal amplification, rapid, high sensitivity, tube or on chip amplification
<li>mass spectroscopy – unequivocal sample discrimination, fast high throughput analyses small amplicon detection
</ul>
<p>This project is now complete and the Agency is currently evaluating the final report.
Current methods for the detection and quantification of genetically modified (GM) ingredients are predominantly ELISA and PCR based techniques that are now relatively standard analytical practice.
<p>However, the increasing demands placed upon trace nucleic acid detection technologies in other fields, such as medical diagnostics and forensic analysis, have driven the development and application of alternative, novel technologies that have a potential to reliably detect single target nucleic acid molecules within challenging matrices.
<p>This need for ultra-sensitive detection is in part driven by current trends in regulation, where limits are often set close to or even below the levels reliably detected by available measurement technology.
<p>The EU 1% limit for detection of genetically modified soya and maize ingredients in processed foods is a good example of where measurement struggles to match regulatory demands. However, such techniques have seldom been applied to issues of food authenticity and trace detection.
<p>This project therefore proposes to examine and develop the application of two of the most promising emerging technologies in the context of the identification and quantification of GM ingredients.
<p>Find more about this project and other FSA food safety-related projects at the <a href="http://www.food.gov.uk/science/research/" target="_blank">Food Standards Agency Research webpage</a>.