NOVEL METHODS TO ENHANCE DETECTION OF EQUINE GENE DOPING AGENTS

Thelong-termgoalis to protect the health and welfare of performance and working horses. In the near term, to evaluate the duration of detection of a common viral vector for gene delivery using established techniques that will validate the development of anovel, more sensitivetesting method that may be used stall-side to improve detection methods.Objectives/Specific AimsAim 1:To develop a low-cost, stall-side test using the CRISPR/Cas system for the rapid detection of gene doping vectors.Aim 2:To determine the time course for detection of rAAV2 DNA sequences and expressed gene in plasma, skeletal muscle, and synovial fluid following intramuscular (IM) and intra-articular (IA) administration comparing CRISPR/Cas with ddPCR and NGS for validation.