Determine the antimicrobial resistance of E. coli from cattle reared with various antibiotic usage regimens. Are there intervention strategies that can reverse antimicrobial resistance?
Approach:
1) Will culture approximately 1000 cattle fecal samples for E. coli; 2) E. coli will be tested for antibiotic sensitivities; 3) Resistant bacteria will be genotypically characterized; and 4) Antibiotic usage practices will be altered and cattle populations re-distributed to reduce antibiotic resistance.