PARTNERSHIP: Development of a nanoliter reactor for digitalization of nucleic acid-based detection platforms with broad application

The aims of the proposal are:Aim 1: Fabrication of a nanoliter digital chip (nano-dChip) for nucleic acid amplification; Aim 2:Development of nano-dChip for the digitalized detection of human noroviruses; Aim 3:Development of nano-dChip for the duplexed digitalized detection of foodborne bacterial pathogens (Salmonella enterica and Listeria monocytogenes); Aim 4:Assessment of the ability of the nano-dChip to detect foodborne bacterial and viral pathogens in crudely processed samples.