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National Agricultural Library
U.S. Department of Agriculture
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  4. Phenotypic and Genotypic Characterization of Salmonella Isolates Recovered from Slaughter Plants

Phenotypic and Genotypic Characterization of Salmonella Isolates Recovered from Slaughter Plants

Objective

To conduct susceptibility testing, pulsed field gel electrophoresis (PFGE), data analysis, and database maintenance on FSIS compliance and baseline study Salmonella isolates for inclusion in the NARMS and USDA VetNet Programs.

More information

Approach: Bacteria will be cloned and a single clonotype will be tested for resistance to a custom made panel of antimicrobials important in human and veterinary medicine using the semi automated broth microdilution sensititre system. The isolate will be maintained frozen at -80C and lyophilized. The isolate will also be subjected to PFGE using a single enzyme digestion. Additional enzyme digestions will be done upon request or if the isolate is identified as part of a human food borne outbreak. Resistance and PFGE data will be analyzed and reports will be generated. A manuscript(s) will be written pending mutual consent by both parties.

Investigators
Cray, Paula
Institution
USDA - Agricultural Research Service
Start date
2009
End date
2009
Funding Source
Agricultural Research Service
Project number
6612-32000-002-11R
Accession number
418318
Categories
Salmonella