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National Agricultural Library
U.S. Department of Agriculture
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  4. Phenotypic and Genotypic Characterization of Salmonella Isolates Recovered from Slaughter Plants (Susceptibility Testing)

Phenotypic and Genotypic Characterization of Salmonella Isolates Recovered from Slaughter Plants (Susceptibility Testing)

Objective

To conduct susceptibility testing, pulsed field gel electrophoresis (PFGE), additional characterizations as necessary, data analysis, and database maintenance on FSIS compliance and baseline study Salmonella isolates for inclusion in the National Antimicrobial Resistance Monitoring System (NARMS) and USDA VetNet Programs.

More information

Approach: <br/>
Bacteria will be cloned and a single clonotype will be tested for resistance to a custom made panel of antimicrobials important in human and veterinary medicine using the semi automated broth microdilution sensititre system. The isolate will be maintained frozen at -80C and lyophilized. The isolate will also be subjected to pulsed field gel electrophoresis (PFGE) using a single or double enzyme digestion. Additional enzyme digestions will be done upon request or if the isolate is identified as part of a human food borne outbreak.

Investigators
Cray, Paula
Institution
USDA - Agricultural Research Service
Start date
2012
End date
2012
Funding Source
Agricultural Research Service
Food Safety Inspection Service
Project number
6612-32000-005-06
Accession number
0423115
Categories
Antimicrobial Resistance
Salmonella
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