Pilot Study on the Application of Whole Genome Sequencing Approach for the Molecular Typing of Foodborne Bacterial Pathogens

Objective

<OL> <LI> To evaluate the ability of PPC to reduce fecal shedding of E. coli O157:H7 in lambs challenged with E. coli O157:H7
<LI> To determine the role of PPC tannin in reducing E coli O157:H7 fecal shedding.
<LI> To determine their kill site of E. coli O157:H7 in digestive tract of ruminant (i.e rumen or large intestine). </OL>
In vitro experiments will also be conducted to assess the effects of PPC and PPC tannins on ruminal fermentation and the mechanism by which PPC tannin affects ruminal microbial populations.

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Expected Benefits:<BR>

By assessing the potential of PPC and PPC tannin for reducing E. coli O157:H7 in experimentally inoculated animals, this study may identify an economically feasible protocol by which livestock producers can implement a pre-harvest strategy to control E. coli O157:H7 in cattle. Proactive approaches to controlling E. coli O157:H7 in beef cattle contribute significantly towards minimizing the risk posed by this zoonotic pathogen to the Canadian food supply.
<P>
Recommendation of an economically feasible dietary intervention strategy which will control E. coli O157:H7 in ruminant will be instrumental in reducing numbers of E. coli O157-positive cattle entering packing plants and minimizing the incidence of foodborne illness associated with this pathogen

Investigators

Wang, Yuxi

Institution

Agriculture and Agri-Food Canada

Start date

2010

End date

2011

Funding Source

Ontario Min. of Agriculture Food and Rural Affairs

Project number

SF6090