PLANT TRANSFORM: DEVELOPING SOYBEAN GENOTYPES WITH REDUCED/REMOVED TRYPSIN INHIBITOR AND LECTIN IN SEEDS

The long-term goal of this proposal is to develop and release soybean varieties and germplasmwith reduced or removed antinutritional factors, while the focus will be on decreasing/removing two of the most undesirable antinutritional factors in soybean seeds: trypsin inhibitors (TI) and lectins (LEC).Supporting objectivesFunctional characterization of the trypsin inhibitors and lectins antinutritional candidate gene using TILLING-by-sequencing+.Gene editing of the highly seed-expressed genes to stack the best two beneficial Kunitz TI (KTI) and LEC alleles.Development of genotypes derived from the mutants with reduced KTI and LEC content through cross and backcross.