PREHARVEST APPLICATION OF A DAIRY-ORIGINATED PROBIOTIC AND A SALMONELLA VACCINE AGAINST EMERGING SALMONELLA OF FOOD SAFETY IMPORTANCE IN POULTRY

Goals:The long-term goal of our research is to develop effective, long-standing, broadly applicable, and poultry gut-friendly anti-Salmonella strategies to improve poultry preharvest safety. Based on the evidence from our previous and ongoing efforts to develop anti-Salmonella strategies with rapid translatable impacts improving poultry food safety, our immediate goal is to determine the efficacy of a unique combination of a dairy-origin probiotic, Propionibacterium freudenreichii subspecies freudenreichii B3523, (P. freudenreichii) and an industry Salmonella-vaccine, against the emerging Salmonella Reading (S. Reading) in turkeys and Salmonella Infantis (S. Infantis) in broilers.Objectives:1) To determine the effect of the combination of P. freudenreichii and Salmonella-vaccine against crop and cecal colonization, cloacal discharge, and dissemination to internal organs of S. Reading in commercial turkeys,2) To determine the effect of the combination of P. freudenreichii and Salmonella-vaccine against crop and cecal colonization, cloacal discharge, and dissemination to internal organs of S. Infantisin commercial broilers, and3) To characterize the effect of the combination of P. freudenreichii and Salmonella-vaccine during health, infection, and intervention in broilers and turkeys using microbiome analysis, histopathology, immunohistochemistry, and intestinal morphometry.