PRESERVATION OF FEMALE GERMLINE IN LIVESTOCK

The overall aim of this project is to establish a protocol for oocyte freezing (vitrification) that results in a high success rate of in vivo embryo development in small ruminants. This aim will be achieved through establishing and building up a state of the art facility for in vitro embryo production in small ruminants and cattle. Creating this laboratory at Fort Valley State University is essential for training students in various skillsof ART and IVP. Acquiring these skills will expand the knowledge and experience for these trainees and open new doors for their future careers.Specific objectives1. To evaluate the viability and nuclear maturation of goat oocytes after exposure to different vitrification solution (Toxicity test)2. To compare viability and in vitro maturation rates of goat oocytes vitrified at GV stages using different cryo-carriers and cryoprotectants3. To test the effect of vitrification of goat oocytes on spindle configuration and mitochondrial activity4. To evaluate the developmental potential of vitrified/warmed goat oocytes following IVF and embryo culture