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National Agricultural Library
U.S. Department of Agriculture
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  4. Protection of Chickens from Avian Influenza (AI) Challenge Following Vaccination with a Recombinant Salmonella Expressing the M2E Gene of AI

Protection of Chickens from Avian Influenza (AI) Challenge Following Vaccination with a Recombinant Salmonella Expressing the M2E Gene of AI

Objective

<OL> <LI> To evaluate protection from highly pathogenic avian influenza (AI) using a novel recombinant salmonella vaccine expressing the M2e gene of AI. <LI> To characterize the serologic response following challenge.

More information

APPROACH: A salmonella-vectored vaccine expressing the M2e gene of avian influenza will be grown at the University of Arkansas. Groups of 8-10, one-day-old chickens will be inoculated with the vaccine. After 28 days, the birds will be transported to SEPRL for challenge with both HPAI H5N1 and LPAI under BSL3Ag conditions. Protective immunity will be evaluated by morbidity/mortality to challenge, with oral and cloacal swabs taken to determine viral shedding. Humoral immunity will be evaluated by virus neutralization.

Investigators
Hargis, Billy; Kapczynski, Darrell
Institution
University of Arkansas
USDA - Agricultural Research Service
Start date
2007
End date
2008
Funding Source
Agricultural Research Service
Project number
6612-32000-051-12S
Accession number
411708
Categories
Salmonella
Commodities
Meat, Poultry, Game