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National Agricultural Library
U.S. Department of Agriculture
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  4. The Relationship Between Fungal Colonization and Don Contamination in Wheat Seeds

The Relationship Between Fungal Colonization and Don Contamination in Wheat Seeds

Objective

Develop useful and practical tools for screening of germplasm for the Kentucky FHB breeding program. Improve the basic understanding of the mechanism of seed infection by F. graminearum and its relation to FHB symptoms and DON production.

More information

Approach: Develop and optimize a protocol for quantification of F. graminearum fungal biomass in developing mature wheat kernels based on the tri5 primers and SYBR-Green quantification using RT PCR in the highly automated and affordable SmartCycler system. Correlate fungal biomass with DON levels in kernels of three different breeding lines from the Kentucky breeding program that are highly resistant, moderately resistant, and susceptible to FHB. Use cytological methods to relate fungal development and localization over time to levels of DON and symptom development in infected seeds from resistant, moderately resistant, and susceptible wheat varieties.

Investigators
Walker-Simmons, M. Kay
Institution
University of Kentucky
USDA - Agricultural Research Service
Start date
2006
End date
2008
Funding Source
Agricultural Research Service
Project number
0500-00053-002-59G
Accession number
410639