Role of the Rcs Two-Component Pathway in Bacterial Growth on Solid Surfaces and Biofilm Formation

Despite the economic importance of biofilms remarkably little is known about how bacteria regulate gene expression during the formation of these surface-associated communities. Recent results suggest that the Rcs two- component pathway in Escherichia coli may play a regulatory role. <P>
The objective of this study is, therefore, to characterise the role of the Rcs two-component pathway in regulating the changes in gene expression associated with the transition from planktonic growth to growth on a solid surface. This objective will be achieved by:<OL> <LI> Genetic and biochemical characterisation of the Rcs two-component pathway. <LI>Identification of the genes controlled by this pathway and genetic analysis of their role in biofilm formation.