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1) Define the immune response associated with acute and chronic Salmonella infection in swine; 2) Determine the effect(s) of transportation stress on the neuroendocrine and immune systems of swine; 3) Identify virulence factor(s) and/or mechanisms important in the pathogenesis of S. choleraesuis and S. typhimurium in swine.
Immune parameters associated with salmonellosis will be measured by bioassay, ELISA, chemiluminescence, and flow cytometry. The interaction between stress, porcine immunity, and Salmonella spp. shedding, will be evaluated using a porcine stress model. Neuroendocrine responses will be measured in collaboration with the Livestock Behavior Research Unit at Purdue University. A bactericidal assay using flow cytometry will be developed and compared with genetic polymorphisms. Porcine mRNA expression will be analyzed using cDNA microarray chip technology. Production of biologically active porcine sTNFR-1 will be optimized. Virulence factors and/or mechanisms underlying pathogenesis of Salmonella spp. (including S. typhimurium DT104) in the gut will be identified using electron microscopy and histological techniques. NADC, Ames, IA, PHFSEDR Lab; Bldg 2, A-5,Barns 109,111,117,126,134: BL-2; Exempt 3/30/99.
Fitzsimmons, C.J., Marklund, L., Stabel, T.J., Galina, L., Tuggle, C.K. Evaluation of cross- species hybridization with porcine mRNA to human microarrays. Proceedings, International Conference Plant and Genome Research, San Diego, CA. 2001. Abstract p.212.Janutenaite, J., Lager, K., Stabel, T., Pesch, B., Brockmeier,S. Comparison of cellular immune response induced by attenuated and wild-type PRRS virus. 2001. 82nd Conf. Res. Workers Anim. Dis. Abstract p. 16.Mwangi, S., Stabel, T. Expression and characterization of the biological activity of a soluble bovine tumor necrosis factor receptor I-IgG fusion protein. 2001. 82nd Conf. Res. Workers Anim. Dis. Abstract p. 18.