Analyze the two different 2-kb promoters derived from genomic clones of the apple alpha-farnesene synthase gene AFS1 to determine the mechanism of ethylene- and cold-induced expression in cold-stored apple fruit. Transform tissue from a scald-susceptible apple cultivar with an RNAi construct of AFS1, regenerate transformed plants, and test the transformants for supression of ethylene-and cold-induced alpha-farnesene synthesis.
SUPPRESSION OF ALPHA-FARNESENE SYNTHESIS FOR NON-CHEMICAL CONTROL OF APPLE SCALD
Objective
Investigators
Whitaker B D
Institution
Cornell University
Start date
2008
End date
2012
Funding Source
Project number
1245-43000-012-02S
Accession number
412635
Categories
Commodities