<OL> <LI> To produce a report reviewing literature transmission of zearalenone (ZON) to eggs, methods of analysis for ZON in eggs, produce final work <LI>
To complete method development, develop suitable robust method with required detection limits, and produce draft standard operating procedure (SOP) in standardised format <LI>
To assess stability of zearalenone and metabolites in eggs during frozen storage. <LI>To complete in-house validation of method <LI>To carry out a trial of the validated method by analysis of twenty samples
A better knowledge and understanding on trichothecene transmission rates to eggs, the form of the compounds present (parent compound, metabolites) and nature of any conjugates is needed. Focus will be placed on deoxynivalenol (DON), nivalenol (NIV), 3-acetyldeoxynivalenol (3-AcDON), 15-acetyldeoxynivalenol (15-AcDON), T2 toxin and HT2 toxin.
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The development of a robust method for low level low or sub parts per billion (ppb) determination of deoxynivalenol, nivalenol, T2 toxin, HT2 toxin in eggs will be carried out.
<p>Find more about this project and other FSA food safety-related projects at the <a href="http://www.food.gov.uk/science/research/" target="_blank">Food Standards Agency Research webpage</a>.