Translational taxonomy: the connection between pathovar designation and genetic diversity with the epidemiology of P. syringae genomospecies 1 causing cucurbit fruit diseases in the United States

The overall goal of this project is to provide an opportunity for a postdoctoral research assistant to develop the necessary skillset needed to transition into a program lead position. The research project will combine traditional lab methods for characterizing an emerging bacterial pathogen (Pseudomonas syringae genomospecies 1) on cucurbits in Washington State with molecular techiques, and field/ greenhouse studies to determine the pathovar designation, and to assess the genetic diversity and epidemiology. The research objectives for this project are to: (i) assess haplotypes and genetic relationships of P. syringae isolates collected in 2015 and 2016 from cucurbits in WWA for comparison with isolates collected from NZ and GA; (ii) identify selected P. syringae isolates obtained from pumpkin leaves, and fruits to pathovar and confirm identity with a leading phytobacteriologist (Dr. Carolee Bull) at PSU; (iii) determine effects of infection times on P. syringae fruit disease incidence and severity; and, (iv) establish the contribution of rain pooled on agricultural mulches, and soilborne inoculum from cucurbit plant residues, as potential sources of secondary inoculum of the pathogen.Soft skills to be developed throughout this project are time management, writing and improved listening skills, as well as mentoring skills.