Use of Immune Repertoire Capture™ Technology to Identify and Characterize Ig repertoires: Bovine Vaccination for Theileria parva 

The Animal Disease Research Unit (ADRU, ARS-USDA) in collaboration with the International Livestock Research Institute (ILRI) and other members of an international consortium, are developing a next generation vaccine against Theileria Parva (East Coast Fever, ECF). Characterizing the immune response in premunized livestock can aid development of the vaccine, and thus, there is interest in applying Atreca’s Immune Repertoire Capture™ (IRC™) technology to sequence the natively paired immunoglobulin genes expressed in single B cells from vaccinated livestock. IRC™ technology involves flow cytometry sorting of blasting B cells, bar-coding and PCR amplification of immunoglobulin cDNA from each single cell, next generation sequence analysis of pooled cDNA, and de-convolution of sequence data to link paired heavy and light chains expressed by single B cells.