Validation of Carbamate Pesticide Screen For Vetlirn Network

CAHFS ABSTRACTThe toxicology section of the California Animal Health and Food Safety Laboratory System issubmitting a grant proposal for the FDA Center for Veterinary Medicine's Funding OpportunityAnnouncement number PA-13-244, titled:' Vet-LIRN Cooperative Agreement Program to Expandand Validate Testing Methods for Food Contaminants in Animal Diagnostic Specimens (U18)'.CAHFS Toxicology is a member of the Vet-LIRN network. We are applying for a full 5 years offunding. We propose that within the first three years of funding we will validate theperformance of a qualitative screening method for carbamate pesticides in feed, rumen (orstomach) content and liver. Carbamates are highly toxic insecticides, with current pesticide useregistrations. They are found both in agricultural and home environments. These matrices areones we commonly encounter in our diagnostic laboratory, and represent the common matricesthat may be collected from an animal exposure situation. During the fourth year we proposevalidating a method for the analysis of Ricinine in liver. Ricinine is a biomarker of thebioterrorism agent Ricin. During the fifth year we propose validating a method for themycotoxins Roquefortine and Penitrem-A in stomach content.For each method we propose following the AOAC guidelines Level Three: Multi-LaboratoryValidation. While these guidelines require that at minimum two labs validate a method, we areproposing at least three labs collaborate in this validation effort. The methods are allqualitative, and are all performed on an LC/MS platform. Performance characteristics of theseinstruments can vary widely due to differences in ion source design between differentmanufacturers. Therefore, having the methods validated on a variety of brands of instrumentswill enhance the robustness of the methods and ensure that they will be widely applicable inother Vet-LIRN member laboratories.