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Determine the stability and chemical fate of chlorate in mixed urine-feces from feedlot cattle. Determine the activity of chlorate salts against E. coli O157:H7 in mixed urine-feces. Determine the stability and chemical fate of chlorate in a simulated (anaerobic) lagoon and in aerobic compost systems fortified with manure from feedlot cattle. Determine ruminal and fecal microbial species responsible for the microbial reduction of chlorate not associated with pathogen elimination.
APPROACH: In vitro studies will be conducted that measures the relative rates of chlorate degradation in soils, in artificial lagoons, and in compost materials. In addition, efficacy of chlorate at killing pathogenic bacteria in soil systems will be determined. Chlorate will be added in sufficient quantities to mimic the amount of chlorate that might be excreted in urine of feedlot animals. Viability studies will be conducted under aerobic and anaerobic conditions. Chlorate-reducing bacteria will be isolated from ruminal fluid or feces from feedlot animals by enrichment techniques.
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PROGRESS: 2005/10 TO 2006/09
Progress Report 4d Progress report. This report serves to document research conducted under Trust Fund Cooperative Agreement #58-5442-6-0416 between ARS and the National Cattlemens Beef Association. Additional details of research can be found in the report for the parent project 5442-32000-012-00D, Metabolic Variables Affecting the Efficacy, Safety, and Fate of Agricultural Chemicals. Initial attempts to isolate a chlorate metabolizing bacteria from ruminal fluid of cattle were not successful. A trial is currently underway to determine the rate of chlorate degradation and the effect of chlorate on Salmonella enterica and E. Coli O147:H7 in aerobic and anaerobic incubated mixed urine and feces.