Research Publications (Food Safety)

Yu, L. S. et al. A method for detection of flumethrin residue in honey by high-performance liquid chromatography was established. After n-hexane–dichloromethane (4:6, vol/vol) extraction, the honey samples were concentrated by rotary evaporation, purified by an Oasis HLB solid-phase extraction column, and detected using a UV detector at 267 nm.

Marshall, Kristin M. et al. The purpose of this study was to determine the effect of sporulation temperature on the resistance of Clostridium botulinum type A spores of strains 62A and GiorgioA to thermal and high pressure processing (HPP). Spore crops produced in Trypticase–peptone–glucose–yeast extract broth at four incubation temperatures (20, 27, 37, and 41°C) were harvested, and heat resistance studies were conducted at 105°C (strain 62A) and 100°C (strain GiorgioA).

Pouillot, Régis et al. The Interagency Risk Assessment—Listeria monocytogenes (Lm) in Retail Delicatessens provides a scientific assessment of the risk of listeriosis associated with the consumption of ready-to-eat (RTE) foods commonly prepared and sold in the delicatessen (deli) of a retail food store. The quantitative risk assessment (QRA) model simulates the behavior of retail employees in a deli department and tracks the Lm potentially present in this environment and in the food.

Xu, Yunfeng et al. Pomegranate rind is rich in tannins that have remarkable antimicrobial activities. This study aimed to evaluate the effects of a tannin-rich fraction from pomegranate rind (TFPR) on Listeria monocytogenes virulence gene expression and on the pathogen's interaction with human epithelial cells. Growth curves were monitored to determine the effect of TFPR on L. monocytogenes growth.

Liu, Nancy T. et al. Biofilm-forming bacteria resident to food processing facilities are a food safety concern due to the potential of biofilms to harbor foodborne bacterial pathogens. When cultured together, Ralstonia insidiosa, a strong biofilm former frequently isolated from produce processing environments, has been shown to promote the incorporation of Escherichia coli O157:H7 into dual-species biofilms. In this study, interactions between E. coli O157:H7 and R.

Liu, Yang et al. This study evaluated the heat and pressure resistance of 112 strains of Escherichia coli, including 102 strains of verotoxigenic E. coli (VTEC) representing 23 serotypes and four phylogenetic groups. In an initial screening, the heat and pressure resistance of 100 strains, including 94 VTEC strains, were tested in phosphate-buffered saline (PBS).

Heyse, Serena et al. Human salmonellosis has been associated with contaminated pet foods and treats. Therefore, there is interest in identifying novel approaches for reducing the risk of Salmonella contamination within pet food manufacturing environments. The use of lytic bacteriophages shows promise as a safe and effective way to mitigate Salmonella contamination in various food products.

Bosilevac, Joseph M. et al. Escherichia coli O157:H7 and Salmonella are significant foodborne pathogens that can be found in the feces and on the hides of meat animals. When hides are removed during the harvest process, the carcass and subsequent meat products can become contaminated. Camels, cattle, sheep, and goats are harvested for meat in Riyadh, Saudi Arabia. The prevalence of E.

Liu, Zongbao et al. This study was conducted to investigate the prevalence and molecular characterization of multidrug-resistant (MDR) Enterobacteriaceae isolated from swine meat and the breeding environment. A total of 102 MDR Enterobacteriaceae strains belonging to five genera were obtained from 210 samples collected from a large-scale swine farm from March 2012 to June 2013 in Xiamen, People's Republic of China.

The occurrence of antimicrobial-resistant bacteria is an important public health issue. The aim of this study was the monitoring of resistant Escherichia coli in raw cow's milk with a focus on the detection of extended-spectrum β-lactamase (ESBL)–producing strains. In total, 263 samples of raw milk from 40 farms were collected and investigated in 2010 to 2013 in the Czech Republic. Detection of E.

Khalid, Mohd Ikhsan et al. The present study was conducted to investigate the prevalence and antibiotic resistance among Campylobacter jejuni in ulam at farms and retail outlets located in Kuala Terengganu, Malaysia. A total of 526 samples (ulam, soil, and fertilizer) were investigated for the presence of C. jejuni and the gene for cytolethal distending toxin (cdt) by using a multiplex PCR method.

Shenge, Kenneth C. et al. Although recent reports indicated that produce contamination with foodborne pathogens is widespread in Nigeria, the sources and magnitude of microbial contamination of fruits and vegetables on farms and in markets have not been thoroughly identified. To ascertain possible pathways of contamination, the frequency and magnitude of coliform and Escherichia coli contamination of tomatoes produced in northwest Nigeria was assessed on farms and in markets.

Balaguero, Alina N. et al. Overhead spray and brush roller (OSBR) treatment has been shown to remove significantly more Salmonella from tomato surfaces than flume treatment. However, OSBR is not widely used in tomato packing facilities compared with other commodities, and little is known about whether brushing causes microabrasions or other physical damage.

Lieberman, Vanessa M. et al. Whole and diced yellow onions (Allium cepa) were inoculated with five-strain cocktails of rifampin-resistant Escherichia coli O157:H7 or Salmonella and stored under conditions to simulate food service or consumer handling. The inoculum was grown in broth (for both whole and diced onion experiments) or on agar plates (for whole onion experiments).

Singh, Randhir et al. The purpose of this study was to determine the gene expression of Escherichia coli O157:H7 heat shocked in dairy compost. A two-step real-time PCR assay was used to evaluate the expression of stress and virulence genes in E. coli O157:H7 heat shocked in compost at 47.5°C for 10 min. Heat-shocked E.

Peng, Shengfeng et al. Eugenol is a major phenolic component with diverse biological activities. However, it is difficult to formulate into an aqueous solution due to poor water solubility, and this limits its application. In the present study, eugenol nanoliposomes (EN) were prepared by combining the ethanol injection method with the dynamic high-pressure microfluidization method. Good physicochemical characterizations of EN were obtained.

Giacometti, Federica et al. Two quantitative risk assessment (RA) models were developed to describe the risk of salmonellosis and listeriosis linked to consumption of raw milk sold in vending machines in Italy.

Palencia, Edwin R. et al.

Nielsen, Kristian Fog et al.In a recent article in Journal of Food Protection, Palencia and co-workers (34) published a survey on black Aspergillus species isolated from maize and peanuts reporting the production of some mycotoxins. An important claim in the article is their strains of Aspergillus niger
produce fumonisins B1 and B3. However, for many reasons, we consider the production of these two fumonisin isomers unlikely.

Davidson, P. Michael et al. Volume 77 of the Journal of Food Protection, published in 2014, contained 2,224 pages and 295 articles, which included 290 research papers (including research notes), and 5 review, general interest, and supplement papers (Table 1). By comparison, Volume 76 published in

Aim
To determine the presence of the T6SS in Campylobacter jejuni from diverse sources.

Publication date: Available online 9 January 2015

Author(s): Hung King Tiong , Steven Hartson , Peter M. Muriana

Azole fungicides have been reported to be the most effective active substances in the control of Fusarium Head Blight (FHB) and in the reduction of the main mycotoxins that occur in cereal grain, such as deoxynivalenol (DON).

Of a total of 143 presumptive yeast isolates obtained from Galician (north-west Spain) short-ripened raw cow's milk starter-free cheeses, 27 exhibited microbial alcohol-conferred hemolytic (MACH) activity. Among the MACH-positive isolates, eight were identified as Yarrowia lipolytica and four as Kluyveromyces lactis. These isolates of potential technological interest were subjected to spot agar assays on special blood agar media prepared with skim milk, whole milk and cheese.

The glucose oxidase (GOD) produced by the fungus Aspergillus tubingensis will be used to improve the quality of bread. The GOD preparation was assessed in a set of toxicological tests to document its safety in use. The oral administration of the GOD preparation to rats at a dose level of 0.4 mg/kg body weight/day did not cause any overt toxic effect on the survival, food intake and body weight gain throughout the 4-week treatment period.