Research Publications (Food Safety)

Biofilm is made up of microbes and their extracellular matrix, making microorganisms highly tolerant, resistant, and resilient to a wide range of antimicrobials. Biofilm treatment with conventional antimicrobial agents can accelerate the evolution and spread of resistance due to the reduced efficacy and increased gene transfer and differentiation within biofilms. Therefore, effective biofilm-targeting compounds are currently highly sought after.

The human colonic mucus is mainly composed of mucins, which are highly glycosylated proteins. The normal commensal colonic microbiota has mucolytic activity and is capable of releasing the monosaccharides contained in mucins, which can then be used as carbon sources by pathogens such as Enterohemorrhagic Escherichia coli (EHEC).

New Delhi metallo-β-lactamase-13 (NDM-13) is an NDM variant that was first identified in 2015 and has not been detected in Salmonella species prior to this study. Here we describe the first identification of a Salmonella Rissen strain SR33 carrying blaNDM-13. The aim of this study was to molecularly characterize SR33’s antimicrobial resistance and virulence features as well as investigate the genetic environment of blaNDM-13.

Microbially induced carbonate precipitation (MICP), a type of urease-based biomineralization, has been a well-researched technique in recent years for heavy metal immobilization; however, the efficiency of the process remains in question. Poly(amino acids) are known to enhance enzymatic activity.

This study investigated the effects of two probiotics, namely Lactobacillus paracasei and Bifidobacterium longum, as feed additives on growth performance, nonspecific immunity, immune-related gene expression, and disease resistance against Vibrio parahaemolyticus in Penaeus vannamei. The experimental diets were prepared using L. paracasei and B.

The goal of the present study was to estimate the distribution and migration of eleven systemic and non-systemic pesticides between the skin and the pulp of apples. Additionally, the behaviour of acetamiprid, boscalid, bupirimate, cyprodinil, flonicamid, fluopyram, pirimicarb, pyrimethanil, thiacloprid, deltamethrin, and fludioxonil in two apple varieties during four thermal processes ranging between −40 °C and 150 °C was discussed.

With the increasing variety of meat analog products, it is important to verify that the methods used for Salmonella detection are effective.

Zearalenone (ZEN) is one of the major agricultural feed contaminants causing serious health hazards worldwide and its degradation by beneficial microbial agents is now being widely examined. In this study we investigated the degradation ability of Klebsiella pneumoniae strain GS7-1 isolated from corn field in Shandong province in China on ZEN. The effects of temperature, incubation time and pH were studied.

Sprouting seeds are considered part of a healthy and nutritious diet, and accordingly, their consumption increases globally. However, edible sprouts are occasionally linked to outbreaks of foodborne diseases, with Salmonella enterica being a frequent contaminant. Biocontrol was recently proposed as a possible approach to combat sprout contamination during production.

Spermidine is an important polyamine that can be used for the synthesis of various bioactive compounds in the food and pharmaceutical fields. In this study, a novel efficient whole-cell biocatalytic method with an NADPH self-sufficient cycle for spermidine biosynthesis was designed and constructed by co-expressing homoserine dehydrogenase (HSD), carboxyspermidine dehydrogenase (CASDH), and carboxyspermidine decarboxylase (CASDC).

Letters in Applied Microbiology, Accepted Article. One of the main drawbacks of chlorine disinfectants is the emergence of chlorine adapted (CA) or resistant microbial cells. This research aimed to investigate the effect of chlorine adaptation on resistance of Salmonella enterica upon atmospheric cold plasma (ACP) application at different voltages (6, 8 and 11 kV) and times (5, 10 and 15 min).

Background Bacterial biofilm can occur on all medical implanted devices and lead to infection and/or dysfunction of the device. In this study, artificial biofilm was formed on four different medical implants (silicone, piccline, peripheral venous catheter and endotracheal tube) of interest for our daily clinical and/or research practice.

Background We investigated the trend change in vancomycin-intermediate Staphylococcus aureus (VISA)/heterogeneous VISA (hVISA) prevalence among methicillin-resistant S.

Increasing groundwater pollution through toxic contamination is the primary concern for severe environmental and health hazards across the world. Groundwater resources are more vulnerable to toxic contamination compared to surface water because they require a long time to restore. Groundwater vulnerability and risk assessment is a challenging task among the researchers responsible for quality monitoring and management of groundwater.

This study aimed to establish a mathematical model capable of predicting the adhesion and biofilm formation by Bacillus cereus isolates on stainless steel surface under different combinations of pH, temperature and time. Logistic regression models were developed to predict the probability of adhesion or biofilm formation on the surface by the pathogen.

Varying culture methods are commonly used for eastern oyster, Crassostrea virginica, aquaculture in the Northeast United States. Vibrio vulnificus and V.

Response of culturable microbes on the surface of apples treated with slightly alkaline electrolyzed water (SAIEW) is largely unexplored. Thus, the aim of this study was to characterize culturable microbes on the surface of SAIEW treated ‘Granny Smith’ apples using conventional and molecular approach. Results showed that SAIEW treatments and storage duration influenced culturable microbes isolated from the surface of ‘Granny Smith’ apples stored at 5 °C for 21 days.

Hazardous chemicals are commonly found in cereals and cereal-based products. However, most studies focus on the individual effects of these mycotoxins or metals, rather than their combined toxicity.

Environmental contamination with Cadmium (Cd) is of great concern due to its hazardous effects on living organisms.Query In the present research, Leucaena leucocephala plants were exposed to Cd concentrations of 5, 10, and 15 mg/L to determine their potential use in Cd remediation. Different parameters including Cd uptake, macro/micronutrient content, chlorophyl, and catalase production were determined. Results indicated that Cd uptake by L.

Salmonella is one of the most common food-borne pathogens, and the food safety problem caused by Salmonella has raised concern globally. In this work, a novel electrochemical biosensor based on single primer isothermal amplification (SPIA) was established for the specific and sensitive detection of Salmonella.

Metal(oid)s contamination in agricultural soils can create adverse environmental conditions and pose human health risks. The present study was conducted to assess metal(oid)s contents, source identification, and ecological and health risks due to metal(oid)s contamination in BISIC industrial region soils of Tangail district Bangladesh.

The integrated management of water resources is a requirement for environmental preservation and economic development, with the removal of nutrients being one of the main drawbacks.

Deoxynivalenol, a mycotoxin that may present in almost all cereal products, can cause huge economic losses in the agriculture industry and seriously endanger food safety and human health. Microbial detoxifications using microbial consortia may provide a safe and effective strategy for DON mitigation.

Journal of Applied Microbiology, Accepted Article. Aims Norovirus remains the most significant virological risk that is transmitted via food and the environment to cause acute gastroenteritis.

by Festus S. Shafodino, Julien M. Lusilao, Lamech M. Mwapagha Nigella sativa is one of the medicinal plant species that gained popularity for a wide range of medicinal applications due to its seeds which are rich in phytoconstituents. Continuous scientific investigations on N. sativa seeds are needed to better understand its many medicinal potentials.