Research Publications (Food Safety)

Salmonella represents a notable food-borne health threat in the European Union, primarily transmitted through poultry meat. Consequently, there is an augmented regulatory emphasis on poultry safety. However, chemical treatments can degrade meat's organoleptic quality, necessitating innovative contamination-reduction strategies. A promising approach for the biological control of food-borne diseases is lytic bacteriophages.

Previous studies have shown that avian hepatitis E virus (HEV) decreases egg production by 10-40% in laying hens, but have not fully elucidated the mechanism of there. In this study, we evaluated the replication of avian HEV in the ovaries of laying hens and the mechanism underlying the decrease in egg production. Forty 150-days-old commercial laying hens were randomly divided into 2 groups of 20 hens each.

The aim of the study was to develop a modified QuEChERS method coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS) for the simultaneous determination of five multi-class pesticides in country beans collected from Dhaka, Bangladesh. Pesticides were extracted using ACN, and to minimize the co-extraction matrix, optimized d-SPE cleanup was done using sorbents (GCB, PSA, and C18).

Nowadays, unprecedented health challenges are urging novel solutions to address antimicrobial resistance as multidrug-resistant strains of bacteria, yeasts and moulds are emerging. Such microorganisms can cause food and feed spoilage, food poisoning and even more severe diseases, resulting in human death. In order to overcome this phenomenon, it is essential to identify novel antimicrobials that are naturally occurring, biologically effective and increasingly safe for human use.

Effect of inoculation protocol on thermal inactivation of Salmonella spp. Abstract The laboratory inoculation techniques should ideally mimic the real‐life environment to reliably estimate the decimal reduction time (D‐value) of bacteria for process validation. This study aims at investigating the influence of the inoculation method on the D‐value of Salmonella in black pepper powder.

Contamination with multiple mycotoxins is a major issue for global food safety and trade. This study focused on the degradation of aflatoxin B1 (AFB1) and zearalenone (ZEN) by 8 types of edible fungi belonging to 6 species, inclulding Agaricus bisporus, Agrocybe cylindracea, Cyclocybe cylindracea, Cyclocybe aegerita, Hypsizygus marmoreus and Lentinula edodes. Among these fungi, Agrocybe cylindracea strain GC-Ac2 was shown to be the most efficient in the degradation of AFB1 and ZEN.

The rumen microbiota and metabolites play an important role in energy metabolism and immune regulation of the host. However, the regulatory mechanism of rumen microbiota and metabolite interactions with host on Tibetan sheep’s plateau adaptability is still unclear. We analyzed the ruminal microbiome and metabolome, host transcriptome and serum metabolome characteristics of Tibetan sheep at different ages.

Recently emerged S. Infantis strains carrying resistance to several commonly used antimicrobials have been reported from different parts of the globe, causing human cases of salmonellosis and with occurrence reported predominantly in broiler chickens. Here, we performed phylogenetic and genetic clustering analyses to describe the population structure of 417 S. Infantis originating from multiple European countries and the Americas collected between 1985 and 2019.

Patulin is a secondary metabolite primarily synthesized by the fungus Penicillium expansum, which is responsible for blue mold disease on apples. The latter are highly susceptible to fungal infection in the postharvest stages. Apples destined to produce compotes are processed throughout the year, which implies that long periods of storage are required under controlled atmospheres. P.

The current study aimed to evaluate aflatoxin M1 (AFM1) contamination in a total number of 240 samples collected during four consecutive seasons (from January to November 2022) from two western Iranian provinces (Ilam and Lorestan). AFM1 was determined by high-performance liquid chromatography with a fluorescence detector after the toxin extraction with immunoaffinity columns. The results showed 100% of AFM1 contamination in all the surveyed matrices.

Microbiology Resource Announcements, Ahead of Print. Nine Peruvian isolates of Vibrio parahaemolyticus were characterized through sequencing, revealing the presence of simple sequence repeat, Pir toxin-like genes, and genes associated with antibiotic resistance, toxic components, and transposable elements. These findings expand our understanding of the genetic diversity, disease resistance, and virulence in cultivated shrimp populations in Peru.

Listeria monocytogenes, a contaminant of raw milk, includes hypervirulent clonal complexes (CC) like CC1, CC4, and CC6, highly overrepresented in dairy products when compared to other food types. Whether their higher prevalence in dairy products is the consequence of a growth advantage in this food remains unknown. We examined growth kinetics of five L.

Peanut kernels could be easily contaminated by Aspergillus flavus and its mycotoxins. This not only reduced their nutritional and economic value, but also posed a serious threat to human health. In this study, acid-modified attapulgite loaded with bacillomycin D (H-ATP-BD) was used for aflatoxin B1 (AFB1) removal in peanut kernels contaminated with A. flavus spores. During storage, H-ATP-BD treatment inhibited mildew of peanut kernels.

There is an increasing awareness in the field of Salmonella epidemiology that focusing control efforts on those serotypes which cause severe human health outcomes, as opposed to broadly targeting all Salmonella, will likely lead to the greatest advances in decreasing the incidence of salmonellosis. Yet, little guidance exists to support validated, scientific selection of target serotypes.

The essential oil is extracted from Cinnamomum verum through Clevenger. The antimicrobial activities of essential oils were researched in this study. CEO exhibited higher antibacterial effects against S. aureus and E. faecalis. Cytoplasmic membrane permeability plays a vital role in the CEO bactericidal action. Summary In developing countries, fresh fruit juices are prepared and sold without ongoing assessment of the quality and safety of mulberry and carrot juices.

Aims Cadmium (Cd) is absorbed by plants into the food chain as a trace pollutant harmful to humans. The rice (Oryza sativa. L) glycosyltransferases (OsGTs) family is a large protein family with over hundreds of members, however, prior studies have failed to identify which OsGTs has a role in plants' response to Cd toxicity.

This study examined the distribution of 37 pesticide residues in different cereal grains (rye, wheat, oat, and barley) and their fractions, including flour, bran, and feed bran. The grains used in the study were sprayed in the field before processing to provide a more accurate representation of the fate of pesticide residues in these fractions. Pesticides were extracted using the QuEChERS method and analyzed by GC-MS/MS and LC-MS/MS.

Abstract Aiming at accelerating the implementation of cumulative risk assessment to pesticide residues, this report describes a two‐step prioritisation analysis, on individual pesticides and on target organ systems, that allows to identify (i) low‐priority substances expected to have a marginal contribution to cumulative risk, and (ii) high priority organ systems to be addressed in future cumulative risk assessments.

Chickens are the primary reservoirs of Campylobacter spp., mainly C. jejuni and C. coli, that cause human bacterial gastrointestinal infections. However, genomic characteristics and antimicrobial resistance of Campylobacter spp. in low- to middle-income countries need more comprehensive exploration. This study aimed to characterize 21 C. jejuni and 5 C.

Salmonella and Campylobacter are common bacterial hazards causing foodborne illnesses worldwide. A large proportion of Salmonella and Campylobacter illnesses are attributed to contaminated poultry products that are mishandled or under cooked. Processing interventions such as chilling and post-chill dip are critical to reducing microbial contamination of poultry.

Strawberry (Fragaria × ananassa Duch.) is a widely favored horticultural crop renowned for its unique taste and flavor. To develop an accurate predictive model for strawberry freshness, colorimetric data, total soluble solids, titratable acidity, and volatile organic compounds (VOCs) were investigated in fully ripe 'Jukhyang' and 'Merry Queen' strawberries. Data measurements were conducted after 0, 4, 8, and 12 days of cold storage (10 °C, 79% RH).

The increasing risk of food poisoning caused by Staphylococcus aureus (S. aureus) contamination has aroused great concern about food safety. Eugenol is highly favored due to its broad-spectrum antibacterial activity and non-drug resistance property. The study aimed to reveal the anti-bacterial and anti-virulence mechanisms of eugenol against S. aureus using phosphoproteomics.

The aim of this work was to study the effect of a recombinant superoxide dismutase (rSOD) from Bacillus pumilus E−1-1-1 regarding the degradation of aflatoxin M1 (AFM1) in model solution. The degradation mechanism was further explored and applied in milk and beer. The Kinetic Momentum and maximal velocity of the rSOD towards AFM1 were 3.6 μg/mL and 1.8 μg/mL/min, respectively. The rSOD-mediated AFM1 degradation product was identified as C12H11O3.

Autoimmune hepatitis (AIH) is a chronic inflammatory disease of the liver that is mediated by autoimmunity and has complex pathogenesis. Its prevalence has increased globally. Since the liver is the first organ to be exposed to harmful substances, such as gut-derived intestinal microbiota and its metabolites, gut health is closely related to liver health, and the “liver-gut axis” allows abnormalities in the gut microbiota to influence the development of liver-related diseases such as AIH.

This study aimed to determine the nPCR-RFLP genotypes of newly obtained T. gondii isolates from human congenital toxoplasmosis cases in Argentina and to determine their allelic profiles for virulence genes ROP18/ROP5. In addition, the ROP18/ROP5 profiles were also determined for previously characterized T. gondii samples. Isolation from congenital toxoplasmosis cases was carried out in mouse bioassay from two placentas (P1 and P2).