Research Publications (Food Safety)

Harmful Algal Blooms (HAB) are complex to manage due to their intermittent nature and their severe impact on the economy and human health. The conditions which promote HAB have not yet been fully explained, though climate change and anthropogenic intervention are pointed as significant factors.

The eggs of black widow spider (L. tredecimguttatus) have been demonstrated to be rich in toxic proteinaceous components. The study on such active components is of theoretical and practical importance. In the present work, using a combination of multiple biochemical and biological strategies, we isolated and characterized the proteinaceous components from the aqueous extract of the black widow spider eggs.

e-First Articles.

Despite effective food safety interventions within abattoirs, Salmonella enterica remains a common contaminant of raw ground beef. Research has recently implicated peripheral lymph nodes (PLNs) as a potential route by which Salmonella contaminates ground beef.

Foodborne Pathogens and Disease , Vol. 0, No. 0.

Foodborne Pathogens and Disease , Vol. 0, No. 0.

Publication date: 16 June 2015
, Volume 203
Author(s): Qian Shen , Kamlesh A. Soni , Ramakrishna Nannapaneni

Publication date: 16 June 2015
, Volume 203
Author(s): Amreeta Sarjit , Gary A. Dykes

The Agr-like quorum sensing (QS) system of Clostridium perfringens controls production of many toxins, including beta toxin (CPB). We previously showed (Vidal et al. Mol Microbiol. 2012, 83 (1): 179-194) that an eight amino acid, AgrD-derived peptide named 8-R upregulates CPB production by this QS system. The current study synthesized a series of small signaling peptides corresponding to sequences within the C. perfringens AgrD polypeptide to investigate the C.

The antibacterial activity and total phenolicTP) content of Agaricus bisporus stipes were assessed using solvent and water extracts to determine its bioactivity. Extraction methods included accelerated solvent extraction (ASE) and hot water followed by membrane concentration. Water extract from ASE had the highest TP of 1.08 gallic acid equivalents (GAE)/g dry weight (DW) followed by ethanol at 0.61 mg GAE/g DW and 0.11 mg GAE/g DW for acetone.

Caprylic acidCA) was evaluated for reducing Escherichia coli O157:H7 and Salmonella on fresh produce. Spinach, romaine lettuce and iceberg lettuce were inoculated with a cocktail of five E. coli O157:H7 or Salmonella strains, air dried for 30 min and then dipped in CA (10, 25 and 50 ppm) or chlorine (50 ppm) for 60 s. Treated leaves were analyzed for E. coli O157:H7 and Salmonella following treatment and during storage at 4C for 14 days.

The Yersinia type III secretion system (T3SS) translocates Yop effector proteins into host cells to manipulate immune defenses such as phagocytosis and reactive oxygen species (ROS) production. The T3SS translocator proteins YopB and YopD form pores in host membranes, facilitating Yop translocation. While the YopD amino and carboxy termini participate in pore formation, the role of the YopD central region between amino acids 150–227 remains unknown.

Publication date: September 2015
, Volume 55
Author(s): Valentina Bernini , Elena Dalzini , Camilla Lazzi , Benedetta Bottari , Stefano Bisotti , Mauro Fontana , Erasmo Neviani

Aims
Develop a multiplex real time PCR method to identify Campylobacter jejuni containing mutations commonly associated with macrolide resistance.

The Campylobacter jejuni capsular polysaccharide is important for virulence and often contains a modified heptose. In strain ATCC 700819 (a.k.a. NCTC 11168), the modified heptose branches off from the capsular backbone and is directly exposed to the environment. We reported previously that the enzymes encoded by wcaG, mlghB, and mlghC are involved in heptose modification.

Publication date: September 2015
, Volume 55
Author(s): Joanna Trafiałek , Matthias Lehrke , Friedrich-Karl Lücke , Danuta Kołożyn-Krajewska , Johann Janssen

Publication date: September 2015
, Volume 55
Author(s): Muhammad Masood , Shahzad Zafar Iqbal , Muhammad Rafique Asi , Noeen Malik

Foodborne Pathogens and Disease Mar 2015, Vol. 12, No. 3: 221-227.
Background: In April 2013, outbreaks of acute gastroenteritis were reported at three schools in Jeonju, South Korea. Epidemiological investigations were performed to characterize the outbreaks and implement appropriate control measures.

Foodborne Pathogens and Disease Mar 2015, Vol. 12, No. 3: 197-202.

In addition to Escherichia coli O157:H7shiga toxin-producing E. coli (STEC) O26 was added to the zero-tolerance adulterant list together with other 5 non-O157 STEC serogroups in 2012. Four farm O26 isolates were used in this study; they were obtained from a on-farm survey study conducted in Alabama. The presence of 3 major pathogenic genes (stx1, stx2, and eaeA) was determined through multiplex polymerase chain reaction (PCR).

Publication date: 16 June 2015
, Volume 203
Author(s): Muriel Guyard-Nicodème , Katell Rivoal , Emmanuelle Houard , Valérie Rose , Ségolène Quesne , Gwenaëlle Mourand , Sandra Rouxel , Isabelle Kempf , Laurent Guillier , Françoise Gauchard , Marianne Chemaly

Publication date: 2 June 2015
, Volume 202
Author(s): C. Bacci , A. Vismarra , C. Mangia , S. Bonardi , I. Bruini , M. Genchi , L. Kramer , F. Brindani

Publication date: 2 June 2015
, Volume 202
Author(s): Catherine Hennechart-Collette , Sandra Martin-Latil , Laurent Guillier , Sylvie Perelle