Research Publications (Food Safety)

Fungicides are applied from plant development to grape production, transport, and storage. Even after fruit processing, residues of these compounds can be present in grape by-products such as wine and juice.

Journal of Separation Science, Accepted Article. A vortex assisted surfactant enhanced emulsification liquid-liquid microextraction based on non-ionic silicone surfactant was successfully developed for the determination of organophosphorus pesticides in food samples coupled to gas chromatography-mass spectrometry.

Escherichia coli is a commensal bacterium and one of the first bacteria to colonize the digestive tract of newborns after birth. It is characterized by great versatility and metabolic flexibility that allows its survival in different niches. The present study aims at analyzing the diversity of E. coli strains isolated from the intestinal microbiota of children aged from 0 to 5 years in the commune of Abomey-Calavi in Benin.

In the age of big data an important question is how to ensure we make the most out of the resources we generate. In this review, we discuss the major methods used in Apicomplexan and Kinetoplastid research to produce big datasets and advance our understanding of Plasmodium, Toxoplasma, Cryptosporidium, Trypanosoma and Leishmania biology.

This study was conducted to evaluate the effects of hydrogen peroxide (H2O2) concentration, treatment time, and dwell time on the efficacy of cold plasma-activated hydrogen peroxide aerosols against bacteria on stem scar and smooth surfaces of tomatoes.

Salmonella is one of the most common Gram-negative pathogens and seriously threatens chicken farms and food safety. This study aimed to establish a multiplex polymerase chain reaction (PCR) approach for the identification of different Salmonella enterica subsp. enterica. The citE2 gene and interval sequence of SPS4_00301–SPS4_00311 existed in all S.

This study aimed to compare the biofilm formation ability of Staphylococcus aureus isolated from a wide range of animals and study the association between biofilm formation and antimicrobial resistance and genetic lineages. A total of 214 S. aureus strains isolated from pets, livestock, and wild animals were evaluated regarding their ability to form biofilms by the microtiter biofilm assay and their structure via confocal scanning laser microscopy.

Cathine is the stable form of cathinone, the major active compound found in khat (Catha edulis Forsk) plant. Khat was found to inhibit major phase I drug metabolizing cytochrome P450 (CYP) enzyme activities in vitro and in vivo. With the upsurge of khat consumption and the potential use of cathine to combat obesity, efforts should be channelled into understanding potential cathine-drug interactions, which have been rather limited.

Journal of Basic Microbiology, EarlyView. Vibrio parahaemolyticus is a common foodborne pathogenic bacterium and drug-resistant strains are now widespread. Phages led by drug-resistant V. parahaemolyticus strains are promising means to decrease the pressure on public health. We isolated a V. parahaemolyticus-specific bacteriophage F23s1 that was active at wide ranges of temperature (30–60°C) and pH (4–10).

This experiment was conducted to evaluate diets containing a high level of corn silage and alfalfa meal in inducing molt and reducing susceptibility to Salmonella Enteritidis (SE) colonization in laying hens.

Oat contains a variety of phenolic compounds, including avenanthramides, which are found only in oats. This study was conducted to establish the quantitative analysis of seven phenolic compounds in oat powders and sprouted oat powders and validate an efficient high-performance liquid chromatography (HPLC) method. All calibration curves represented good linearity (R2 ≥ 0.9997) in the concentration range (0.5−50 mg/kg) with LOD and LOQ of 0.01−0.21 and 0.02−0.64 mg/kg, respectively.

We assessed the occurrence of aflatoxin M1 (AFM1) in urine in a sample of the MAUCO population-based cohort (n = 120) using an enzyme-linked immune sorbent assay (ELISA) kit specially designed for the analysis of AFM1 in urine. We found AFM1 in the urine of 59% of the participants (> limit of detection), with 12% of the samples being over the limit of quantification.

Naegleria fowleri can cause acute primary amoebic encephalitis. It is known that contact-dependent pathogenicity in free-living amoeba may be mediated through a carbohydrate-dependent pathway. In this study, the effect of mannose on the interaction between N. fowleri and pathogenic Escherichia coli O157:H7 and non-pathogenic E. coli DH5α was analyzed. In particular, the changes in proteases expressed by N.

A simple, rapid and ultrasensitive visual sensing method for the detection of Cronobacter sakazakii (C. sakazakii) based on a biohybrid interface was established. During the entire sensing process, quadruple-cascade amplification showed its superior sensing performance. First, the prepared immunomagnetic beads (IMB) were used to isolate and enrich specific targets from the food matrix.

Sturgeon populations worldwide are threatened with extirpation but little is known about their tendency to bioaccumulate contaminants and their sensitivities to environmental burdens of these contaminants.

The aim of this study was to establish a rapid and visual nucleic acid isothermal amplification method for the detection of viable Staphylococcus aureus (S. aureus) in pork and pork products. The method was based on saltatory rolling circle amplification (SRCA) combined with propidium monoazide (PMA). Eighteen S. aureus strains and 29 non-S. aureus strains were used for the specificity evaluation.

Phage DNA analysis gives opportunity to understand living ecosystem of the environment where the samples are taken. In the present study, we analyzed phage DNA obtained from wastewater sample of university hospital sewage. After filtration, long high-speed centrifugation was done to collect phages. DNA was extracted from pellet by phenol chloroform extraction and used for NGS sequencing.

This is a culture-dependent study with the objective of pure culturing and characterizing pathogenic bacteria from the blowhole, lung, stomach and fecal samples of a neonatal crucially endangered Yangtze finless porpoise (Neophocaena asiaeorientalis asiaeorientalis) that died 27 days after birth. Bacteria were inoculated using a swab onto blood and MacConkey agar plates and representative isolates were identified through 16S rRNA gene sequence analysis.

The prevalence of Shiga-toxigenic E. coli (STEC), harbouring multidrug-resistant genes in raw milk collected from household vendors and cooperative milk marketing societies in Ernakulam District, Kerala, India, has been investigated. A higher prevalence of STEC pathotypes, with 47.16% (20 out of 42 samples), was observed in raw milk. A total of 157 STEC isolates were identified, which included notorious pathoserotypes, E. coli O157 group (10.19%) and E.

In this study, pumpkin seedlings were subjected to cadmium stress (100 mg/L cadmium ion solution, 10 days) without or with wheat straw biochar at different concentrations (0%, 0.5%, 1%, and 2% w/v). As the biochar concentration increased, the amount of cadmium accumulated in the root and stem of pumpkin seedlings decreased and the fresh weight of root, stem and leaf increased. The highest cadmium concentration was in the root, followed by the stem and then the leaf.

It is crucial that a highly effective adsorbent can be used to simultaneously remove the composite pollution including both inorganic and organic arsenic from wastewater. In this work, the iron modified corncob biochar (MCCB), prepared via the co-precipitation of ferric chloride hexahydrate (FeCl3⋅6H2O) with sodium hydroxide (NaOH) on corncob biochar, was studied for the high efficiency removal of arsenilic acid (ASA) and arsenate [As(V)] in wastewater.

Long-term pig manure addition has been widely applied in red soil to improve soil fertility. However, the influence of combined utilization of pig manure and effective microbes (EM) on soil organic carbon (SOC) and Cd are not well understood.

In this study, we developed an efficient method based on magnetic Fe3O4-modified graphene oxide (MNPs@GO) along with aptamer-functionalized upconversion nanoparticles (UCNPs) for Staphylococcus aureus detection. MNPs@GO are prepared using aptamer-functionalized MNPs and GO by means of π-π stacking interaction. In the absence of Staphylococcus aureus, MNPs@GO are formed and are magnetically separated.

The bioactive ingredients of green coffee beans were extracted using high pressure-assisted extraction (HPE) and compared against those derived from the traditional heat reflux (HR) extraction method.

The higher moisture level (70–80%) in wet brewer’s grain (WBG) poses two major difficulties when using it as a feed for different classes of animals. Firstly, transport of WBG is costly. Secondly, the rich polysaccharide and protein content and the high moisture content of WBG make it susceptible to microbial growth and spoilage. Therefore this study was planned to examine the effect of soaking, sun drying and ensiling on nutritional and fungal load dynamics of wet brewers’ grain.