Research Publications (Food Safety)

A double signal enhanced surface plasmon resonance (SPR) biosensor system was developed, which involved graphene-based magnetic dispersion solid-phase extraction (MDSPE) and carboxylated graphene oxide (GO-COOH) materials as amplifying elements in order to achieve real-time determinations of estradiol (E2), diethylstilboestrol (DES), and bisphenol A (BPA) via an indirect competition method.

Clostridium botulinum Group I and Clostridium sporogenes are closely related bacteria responsible for foodborne, infant and wound botulism. A comparative genomic study with 556 highly diverse strains of C. botulinum Group I and C. sporogenes (including 417 newly sequenced strains) has been carried out to characterise the genetic diversity and spread of these bacteria and their neurotoxin genes. Core genome single-nucleotide polymorphism (SNP) analysis revealed two major lineages; C.

The aim of the study was to propose a more efficient and safer botulinum toxin type A (BoNT-A) injection method for the masseter by comparing the conventional blind injection and a novel ultrasonography (US)-guided injection technique in a clinical trial. The 40 masseters from 20 healthy young Korean volunteers (10 males and 10 females with a mean age of 25.6 years) were included in this prospective clinical trial.

Priority pollutants chlorophenols are broadly used chemicals that are persistent in the environment and causing serious human health hazards. The current study introduces a novel adsorbent for the extraction of chlorophenols from river water, surface water, and milk by headspace solid phase microextraction coupled with GC.

Salmonella enterica serovar Typhimurium (S. Typhimurium) is exposed to biotic and abiotic stresses. The survival of Salmonella in nature depends on the global regulators like cAMP receptor protein (CRP). The role of CRP in the phenotypic characteristics and stress tolerance was elucidated in S. Typhimurium using a crp gene null mutant (Δcrp).

Aims To identify and evaluate the application of two novel monoclonal antibody (mAb) 2G12 against outer membrane protein (Omp) C and mAb 12B1 targeting the O chain of the lipopolysaccharides (LPS) of Escherichia coli O157:H7 (ECO157). Methods and Results The sensitivity and specificity of these two antibodies were evaluated with eight ECO157 strains and 68 untargeted strains.

BACKGROUND Olive oil industry generates significant amounts of semi‐solid wastes, namely the olive pomace. Olive pomace is a by‐product rich in high‐value compounds (e.g. dietary fibre, unsaturated fatty acids, polyphenols) widely explored to obtain new food ingredients. However, conventional extraction methods frequently use organic solvents, while novel eco‐friendly techniques have high operational costs.

Some snails (Achatina spp) can be used as a biosensor of heavy metal poisoning. This study thus estimated some heavy metal levels, antioxidant markers, and lipid profiles of snails handpicked around cement factory vicinities in Ogun State, Nigeria. Snails and soil samples were collected from Oke, Ewekoro, Papalanto, and Mowodani Imeko-Afon (control site).

Aim:Bisphenol-A (BPA) is an endocrine disrupting compound and may exacerbate or induce allergic diseases. To the best of our knowledge, there is little evidence regarding the effects of BPA exposure on allergic rhinitis (AR) in children. In the present study, we sought to examine whether exposure to BPA in children is associated with AR.Methods:This study was designed as a case controlled clinical study.

Waste Printed circuit boards (PCBs) are one of the most valuable and recycled components of electronic waste due to the presence of precious metals such as copper, silver, gold and palladium. The rejects of the PCB recycling process, named non-metal fraction (NMF) have continuously been sent to landfills. Several researchers have proposed alternative use of NMF as secondary materials such as fillers in composites or as adsorbent.

Food safety has always been critical to public health and the economic development of civilization. In the United States, the exposé by Upton Sinclair on the conditions in meat-packing houses in a book entitled The Jungle was the primary impetus that resulted in the passage of the Meat Inspection Act and the Pure Food and Drug Act of 1906; this activity established the Bureau of Chemistry which in 1930 was renamed the Food and Drug Administration (FDA).

Due to the importance of a rapid determination of patients infected by multidrug resistant bacteria, we evaluated two rapid diagnostic tests for the detection of third-generation cephalosporins (3GC)-resistant Enterobacterales directly from positive blood cultures within 1 h: BL-REDTM (electrochemical method) and β-LACTATM test (chromogenic method).

Background:Toxoplasma gondii is a parasite of worldwide importance but its burden in indigenous communities remains unclear. In French Guiana, atypical strains of T. gondii originating from a complex rainforest cycle involving wild felids have been linked to severe infections in humans. These cases of Amazonian toxoplasmosis are sporadic and outbreaks are rarely described. We report on the investigation of an outbreak of acute toxoplasmosis in a remote Amerindian village.

We compared the consistency, accuracy and reproducibility of next-generation short read sequencing between ten laboratories involved in food safety (research institutes, state laboratories, universities and companies) from Germany and Austria. Participants were asked to sequence six DNA samples of three bacterial species (Campylobacter jejuni, Listeria monocytogenes and Salmonella enterica) in duplicate, according to their routine in-house sequencing protocol.

by Jun-feng Zhang, Bai Wei, Se-Yeoun Cha, Ke Shang, Hyung-Kwan Jang, Min Kang

by Roderick Salvador, Neil Tanquilut, Rosemarie Macmac, Kannika Na Lampang, Warangkhana Chaisowwong, Dirk Pfeiffer, Veerasak Punyapornwithaya

by Yanan Li, Ren Wang, Zhengxing Chen, Xiuping Zhao, Xiaohu Luo, Li Wang, Yongfu Li, Fei Teng

Non-aureus staphylococci (NAS) are predominantly isolated from bovine milk samples of quarters suffering from subclinical mastitis. They are also abundantly present on dairy cows' teat apices and can be recovered from bovine fecal samples, as recently described. Differences in ecology, epidemiology, effect on udder health, and virulence or protective traits have been reported among the species within this group.

Predictions of drug residues in milk are critical in food protection and are a major consideration in the economics of treatment of mastitis in dairy cows. Nonlinear mixed-effects modeling (NLME) has been advocated as a suitable pharmaco-statistical method for the study of drug residues in milk. Recent developments in physiologically based pharmacokinetic (PBPK) modeling of intramammary drugs allow the combination of a mechanistic description of milk pharmacokinetics with NLME methods.

by Yan Corrêa Rodrigues, Ismari Perini Furlaneto, Arthur Henrique Pinto Maciel, Ana Judith Pires Garcia Quaresma, Eliseth Costa Oliveira de Matos, Marília Lima Conceição, Marcelo Cleyton da Silva Vieira, Giulia Leão da Cunha Brabo, Edilene do Socorro Nascimento Falcão Sarges, Luana Nepomuceno Godim Costa Lima, Karla Valéria Batista Lima

Authentication of dairy and meat products is important to ensure fair competition, consumer benefit, and food safety. The large difference in price between camel and cow milk may be an incentive to adulterate camel dairy products with cow-derived foodstuffs. However, no studies so far have used triplex real-time PCR with an endogenous control to identify camel and cow origins in dairy and meat products.

Author(s): Xu Zhang, Li Ling, Zhiyong Li, Jufang Wang

Author(s): Jungtae Na, Esther Lee, Yu-jin Kim, Mi Ji Choi, Su-Young Kim, Jeong Sun Nam, Bum Jin Yun, Beom Joon Kim

To reduce the use of conventional chemical pesticides, naturally occurring biopesticides such as bacteriophages have emerged as a promising solution, but effectiveness of these biopesticides can be limited because of their UV and desiccation instability. This study developed a biopolymer formulation to improve the phage stability, enhance the antimicrobial activity of phages, and prevent bacterial contaminations on a leaf surface in the presence of UV-A.

Staphylococcal enterotoxins are the most common cause of foodborne intoxications (staphylococcal food poisoning) and cause a wide range of diseases. With at least six variants staphylococcal enterotoxin C (SEC) stands out as particularly diverse amongst the 25 known staphylococcal enterotoxins. Some variants present unique and even host-specific features.